Mi-2 detection for specific PM/DM diagnosis :

With various roles in body like DNA- binding, barrier to transcriptional activation, regulation of developmental processes and skin development and repair of basal epidermis, Mi-2 is considered to be a very important antigen for specific PM/DM diagnosis.

  • Mi-2 is "Myositis-Specific Antibody"(MSA) (It is extremely rare to find these antibodies in patients without myositis, even if they have another muscle or autoimmune disease.)
  • MSA are found almost exclusively in patients with DM/PM and associated overlap syndromes
  • This antibody is associated with Dermatomyositis without other associated connective tissue disorders.
  • 15-20 % Dermatomyositis with a high diagnostic specificity. 95 % of patients with Mi-2 antibodies suffer from dermatomyositis.
  • 15-20 % Dermatomyositis with a high diagnostic specificity. 95 % of patients with Mi-2 antibodies suffer from dermatomyositis.

Significance of Ku detection:

As postulated, Ku antibodies could be found in 55% overlap PM/Systemic sclerosis patients, it is considered to be very important antigen. The anti-Ku antibodies are reported in various connective tissue diseases and the Ku complex can be responsible for a very strong autoimmune answer in autoimmune diseases.

Ku antibodies occurrence:
  • 5-25 % :- Polymyositis and scleroderma overlap-syndrome .

  • 1-7 % :- Myositis .

  • 20 % :- Primary pulmonary hypertension

  • 5-10 % :- SLE .

  • 20 % :- Primary Sjögren’s syndrome
ANA Blot with Myositis Profile
Do not miss Myositis Profile while screening for ANA

AESKUBLOTS ® ANA-17 Pro (REF 4001)

24 tests/kit
Conjugate: anti-human IgG HRP
Color-coded (orange) test strips with cut-off and positive control
Immunoblot for the qualitative detection of IgG antibodies against:

  • dsDNA, nucleosomes, histones, SmD1, PCNA, P0, SS-A/Ro60kD, SS-A/Ro52kD, SS-B/La, CENP -B, Scl-70, U1 snRNP, AMA M2, Jo-1, Pm-Scl, Ku and Mi-2 in human serum or plasma.

AESKUBLOTS ANA-17 Prois used for the differential diagnosis of systemic rheumatic diseases. The detection of autoantibodies in AESKUBLOTS with the corresponding specific antigens allows an easier and more reliable differentiation of specific ANAs (anti-nuclear antibodies). ANAs occur in active and inactive systemic lupus erythematosus (SLE), mixed connective tissue diseases (MCTD), scleroderma, Sjogren's syndrome, primary biliary cirrhosis (PBC) and polymyositis. ANA-17 Pro antigens are fitted on the test strip according to their relevance for the individual autoimmune disease (SLE, Sjogren's syndrome, CREST syndrome, scleroderma, MCTD, myositis, and PBC) for easier interpretation.

For easy interpretation of the AESKUBLOTS, AESKU. DIAGNOSTICS provides the dedicated evaluation software AESKUSCAN.